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prostate epithelial cell rwpe1  (ATCC)
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ATCC prostate epithelial cell rwpe1
Figure 1. Alix, TSG101 and HSC70 protein levels were upregulated in exosomes derived from PCa cells and PCa patient serum. (a) Electron microscopic images of exosomes isolated from PCa cells. (b-e) The protein levels of Alix, TSG101 and HSC70 in the exosomes of normal prostatic epithelial cells (BPH1 and <t>RWPE1)</t> and PCa cells (VCap and LNCap, DU145, PC3) were determined by Western blot assay. *P < 0.05 compared with BPH1-Exo; #P < 0.05 compared with RWPE1-Exo group. (f and g) The protein levels of Alix, TSG101 and HSC70 were measured in the exosomes derived from serum of healthy volunteers (n = 10) and PCa patients (n = 10) with Western blot assay. *P < 0.05 compared with normal-Exo group. N = 10, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data.
Prostate Epithelial Cell Rwpe1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Alix, TSG101 and HSC70 protein levels were upregulated in exosomes derived from PCa cells and PCa patient serum. (a) Electron microscopic images of exosomes isolated from PCa cells. (b-e) The protein levels of Alix, TSG101 and HSC70 in the exosomes of normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cells (VCap and LNCap, DU145, PC3) were determined by Western blot assay. *P < 0.05 compared with BPH1-Exo; #P < 0.05 compared with RWPE1-Exo group. (f and g) The protein levels of Alix, TSG101 and HSC70 were measured in the exosomes derived from serum of healthy volunteers (n = 10) and PCa patients (n = 10) with Western blot assay. *P < 0.05 compared with normal-Exo group. N = 10, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Exosomal lncAY927529 enhances prostate cancer cell proliferation and invasion through regulating bone microenvironment.

doi: 10.1080/15384101.2021.1992853

Figure Lengend Snippet: Figure 1. Alix, TSG101 and HSC70 protein levels were upregulated in exosomes derived from PCa cells and PCa patient serum. (a) Electron microscopic images of exosomes isolated from PCa cells. (b-e) The protein levels of Alix, TSG101 and HSC70 in the exosomes of normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cells (VCap and LNCap, DU145, PC3) were determined by Western blot assay. *P < 0.05 compared with BPH1-Exo; #P < 0.05 compared with RWPE1-Exo group. (f and g) The protein levels of Alix, TSG101 and HSC70 were measured in the exosomes derived from serum of healthy volunteers (n = 10) and PCa patients (n = 10) with Western blot assay. *P < 0.05 compared with normal-Exo group. N = 10, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data.

Article Snippet: Cell culture and cell treatment In this study, human benign prostatic hyperplasia cells BPH1 (# CL0350) and immortalized prostate epithelial cell RWPE1 (#CL0343) were purchased from Fenghui biological Co., Ltd. (Changsha, Hunan, China); human PCa cells VCap (# bncc341358), LNCaP (# bncc261381), DU145 (# bncc338240) and PC3 (# BNCC337715) were purchased from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Derivative Assay, Isolation, Western Blot

Figure 2. Expression of lncAY927529 in exosomes secreted by different prostate cells, prostate cells and clinical serum samples. (a) RT-qPCR was used to detected the lncAY927529 level in the exosomes of normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cells (VCap, LNCap, DU145 and PC3). *P < 0.05 compared with BPH1-Exo; #P < 0.05 compared with RWPE1-Exo group. (b) RT-qPCR was used to detected the lncAY927529 level in normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cells (VCap and LNCap, DU145, PC3). *P < 0.05 compared with BPH1; #P < 0.05 compared with RWPE1 group. (c) The lncAY927529 level in the exosomes of serum of healthy volunteers (n = 10) and PCa patients (n = 10). *P < 0.05 compared with normal serum-Exo group. N = 4, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Exosomal lncAY927529 enhances prostate cancer cell proliferation and invasion through regulating bone microenvironment.

doi: 10.1080/15384101.2021.1992853

Figure Lengend Snippet: Figure 2. Expression of lncAY927529 in exosomes secreted by different prostate cells, prostate cells and clinical serum samples. (a) RT-qPCR was used to detected the lncAY927529 level in the exosomes of normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cells (VCap, LNCap, DU145 and PC3). *P < 0.05 compared with BPH1-Exo; #P < 0.05 compared with RWPE1-Exo group. (b) RT-qPCR was used to detected the lncAY927529 level in normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cells (VCap and LNCap, DU145, PC3). *P < 0.05 compared with BPH1; #P < 0.05 compared with RWPE1 group. (c) The lncAY927529 level in the exosomes of serum of healthy volunteers (n = 10) and PCa patients (n = 10). *P < 0.05 compared with normal serum-Exo group. N = 4, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data.

Article Snippet: Cell culture and cell treatment In this study, human benign prostatic hyperplasia cells BPH1 (# CL0350) and immortalized prostate epithelial cell RWPE1 (#CL0343) were purchased from Fenghui biological Co., Ltd. (Changsha, Hunan, China); human PCa cells VCap (# bncc341358), LNCaP (# bncc261381), DU145 (# bncc338240) and PC3 (# BNCC337715) were purchased from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Expressing, Quantitative RT-PCR

Figure 3. LncAY927529 level was detected in ST2 cells treated with exosomes derived from different PCa cell lines, and PC3 cell proliferation, invasion and apoptosis were measured treated with ST2-CM pretreated with PC3-Exo and DU145-Exo. (a) ST2 cells were treated with exosomes derived from normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cell lines (VCap, LNCap, DU145 and PC3), and RT-qPCR assay was used to detect lncAY927529 level in ST2 cells. *P < 0.05 compared with control group; #P < 0.05 compared with BPH1-Exo group; &P < 0.05 compared with RWPE1-Exo group. (b) LncAY927529 level in PC3 cells was measured treated with ST2-CM pretreated with PC3-Exo and DU145-Exo, which was detected with RT-qPCR assay. *P < 0.05 compared with control conditioned medium of ST2 cells (ST2-CM) group. CCK-8 and Transwell assays were used to detect proliferation (c), invasion (d) and apoptosis (e) of PC3 cells treated with ST2-CM pretreated with PC3-Exo and DU145-Exo. *P < 0.05 or **P < 0.01 compared with ST2-CM group. N = 4, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data.

Journal: Cell cycle (Georgetown, Tex.)

Article Title: Exosomal lncAY927529 enhances prostate cancer cell proliferation and invasion through regulating bone microenvironment.

doi: 10.1080/15384101.2021.1992853

Figure Lengend Snippet: Figure 3. LncAY927529 level was detected in ST2 cells treated with exosomes derived from different PCa cell lines, and PC3 cell proliferation, invasion and apoptosis were measured treated with ST2-CM pretreated with PC3-Exo and DU145-Exo. (a) ST2 cells were treated with exosomes derived from normal prostatic epithelial cells (BPH1 and RWPE1) and PCa cell lines (VCap, LNCap, DU145 and PC3), and RT-qPCR assay was used to detect lncAY927529 level in ST2 cells. *P < 0.05 compared with control group; #P < 0.05 compared with BPH1-Exo group; &P < 0.05 compared with RWPE1-Exo group. (b) LncAY927529 level in PC3 cells was measured treated with ST2-CM pretreated with PC3-Exo and DU145-Exo, which was detected with RT-qPCR assay. *P < 0.05 compared with control conditioned medium of ST2 cells (ST2-CM) group. CCK-8 and Transwell assays were used to detect proliferation (c), invasion (d) and apoptosis (e) of PC3 cells treated with ST2-CM pretreated with PC3-Exo and DU145-Exo. *P < 0.05 or **P < 0.01 compared with ST2-CM group. N = 4, data were expressed as mean ± SEM; Student’s t test or one-way ANOVA was used for analyzing data.

Article Snippet: Cell culture and cell treatment In this study, human benign prostatic hyperplasia cells BPH1 (# CL0350) and immortalized prostate epithelial cell RWPE1 (#CL0343) were purchased from Fenghui biological Co., Ltd. (Changsha, Hunan, China); human PCa cells VCap (# bncc341358), LNCaP (# bncc261381), DU145 (# bncc338240) and PC3 (# BNCC337715) were purchased from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Derivative Assay, Quantitative RT-PCR, Control, CCK-8 Assay